This study determined the properties of 3.2 mm Medium Density Fiberboard (MDF) from a commercial production line. The mixture of fibers from Sesbania aculeate (80%) and Tamarix aphylla (20%) was bound with urea formaldehyde for laboratory testing. Effects of production line speed, fiber moisture content, and production shift on board properties were assessed. Physical and mechanical properties of the panels were determined according to European standards. This study showed that the production of MDF during the evening shift was ideal, with a line speed of 6.00 m/min and fiber moisture content of 8.70 %, compared to morning and night shifts. The production line speed, fiber moisture content, and operation shift affected the properties of MDF boards but met the minimum requirements in the standards. The mix of fibers is a viable combination for commercial production.