Original Article |
2004, Vol.26, No.6, pp. 837-847
Preparation of Newcastle disease virus fluorescent conjugate by immunization of chicken
Pornthip Prommuang, Chongmas Antarasena, and Naruepol Promkuntod
pp. 837 - 847
Abstract
The fluorescent conjugate for diagnosis of Newcastle disease was prepared in 6-week-old chickens by immunization with Newcastle disease virus (NDV), strain F and local isolated strain NK1180/42. The antisera with high neutralizing antibodies titers were collected and pooled.The immunoglobulin was fractionated and conjugated with the fluorescein isothiocyanate (FITC). The conjugate was tested for the specificity in NDV infected chorioallantoic membrane (CAM) and cryostat sections of tracheal epithelium, lung, heart, kidney, spleen, liver, Bursa of Fabricius, brain and intestine. A distinctive fluorescence was seen in the cytoplasm of infected cells of trachea, spleen, Bursa of Fabricius and intestine 48-72 hours after innoculation. The prepared conjugate was specific to NDV and diagnosis and result could be made in 1-2 hours.