Chutima Tippayakul, Sunanta Pongsamart, and Maneewan Suksomtip

pp. 291 - 300

Abstract

Lipid entrapment property of polysaccharide gel (PG) extracted from fruit-hulls of durian (Durio
zibethinus Murr. Cv. Mon-Thong) was investigated in vitro by semi- permeable membrane dialysis technique
using both cellulose membrane and gut sacs of disected jejunum of rat. Lipids (cholesterol, oleic acid and
stearic acid) were mixed with 0-2%W/V PG in the presence of bile salt as a surface active agent in dialysis
membrane. Lipids inside and outside dialysis membrane were analyzed by HPLC method after 4-16 hours
of dialysis in Ringer lactate buffer pH7. Increasing PG concentration resulted in increasing lipids trapped
inside membrane and decreasing lipids released outside membrane. Two percent PG trapped about 80-90%
cholesterol. The result of PG trapping cholesterol in egg york showed that egg york cholesterol released
outside membrane was decreased with increasing PG concentration. A significant relationship was found
between the decreasing of absorbed cholesterol into everted rat jejunum with respect to increasing concentration of PG. These results suggested that durian polysaccharide gel is able to entrap lipids and it seems to
have potential use as medicinal dietary food for lipid controlling patient. Furthermore, in vitro study using
cellulose semi- permeable membrane dialysis method may be applied for preliminary evaluation of polysaccharide effecting lipids absorption.