Supinya Tewtrakul, Supreeya Yuenyongsawad, Sopa Kummee, and Latthya Atsawajaruwan

pp. 503 - 507

Abstract

Volatile oil of dried rhizome of Kaempferia galanga obtained by water distillation was determined for
its chemical components using gas chromatography and mass spectrometry (GC-MS). The major chemical
constituents were identified as ethyl-p-methoxycinnamate (31.77%), methylcinnamate (23.23%), carvone
(11.13%), eucalyptol (9.59%) and pentadecane (6.41%), respectively. Antimicrobial activity of the volatile
oil was tested against various microbes using agar disc diffusion method with the inhibition zones from 8.0-
31.0 mm. Brine shrimp toxicity of volatile oil exhibited an EC₅₀ value of 26.84 µg/ml; whereas the volatile oil
was inactive for antioxidant activity (IC₅₀ >100 µg/ml).