Chatchai Ngamriabsakul and Jiranan Techaprasan

pp. 49 - 57

Abstract

New primers were designed to amplify cpDNA intergenic petA-psbJ sequences in Boesenbergia species. These primers were petA-F, psbJ-R, psbL-R. The aligned sequences of 18 ingroup taxa and 5 outgroup taxa resulted in 856 characters in length, including 8 parsimony informative indels. However, there were only 14
informative characters (~1.65%) in the aligned sequences. The percentage of phylogenetically informative sites was close to the other two regions in Boesenbergia; matK and psbA-trnH. Boesenbergia species form a monophyletic clade with moderate support (81% bootstrap value) in parsimony and UPGMA analyses. Two subclades are recognized and supported by two different basic chromosome numbers. An unpublished new species, Boesenbergia bambusetorum is grouped within the clade of the other two populations of B. longiflora
with weak support (67% bootstrap value). More study is needed to verify the status of the taxon.