Wichada Sunpui and Kamnoon Kanchanapoom

pp. 357 - 364

Abstract

A regeneration procedure was developed for in vitro grown African violet (Saintpaulia ionantha
Wendl.). Adventitious shoot regeneration was compared among petioles, leaves and stems cultured on MS
basal medium. High frequency regeneration was obtained from leaves and petioles supplemented with
plant growth regulators (BA, KN, 2,4-D, and NAA). Regeneration media that induced the highest number of
shoots in petiole explants was either MS medium supplemented with 3 mg/l BA alone or 1 mg/l NAA plus
3 mg/l BA. Adventitious shoots were also regenerated from callus that was derived from leaf explants
cultured on MS medium containing several concentrations of TDZ. Callus induced on medium with 0.5 mg/l
TDZ showed the best shoot induction. Regenerated shoots could be rooted on ½ MS medium or MS medium
supplemented with 3 mg/l IBA. Complete plantlets were acclimatized and successfully transplanted to
glasshouse conditions.