Atikom Riksabutr and Panupong Nuchum

pp. 365 - 369

Abstract

Plantlet regeneration from tissue culture of gnetum could be induced using two pathways
depending upon explant types. Leaf lamina yielded somatic embryos through somatic embryogenesis in
basal MS supplemented with the same concentration of BA and TDZ at 0.5 mg/l. Using cluster of flowers or
ovules in the same medium formula, however, callus formation took place and developed into meristemoid
structures. These calli are called meristematic nodular calli and the pathway was organogenesis. Both
pathways of plantlet regeneration could be applied for mass propagation of gnetum on a commercial scale
in the future.