Pianpan Phiraphinyo, Sutachai Taepakpurenat, Paungkaew Lakkanatinaporn, Worapot Suntornsuk, and Leena Suntornsuk

pp. 327 - 335

Abstract

Physical and chemical properties of two bones of two species of fish, hoki (Macruronus novaezelandiae) and giant seaperch (Lates calcarifer Bloch.), were compared with chicken bone to evaluate their composition
for use as natural calcium supplement. The information could be useful for waste utilization in the food and pharmaceutical industries. Physical testing and chemical analyses were performed according to the USP 24 and BP 1998 standards under calcium carbonate monograph. Loss on drying found in hoki, giant seaperch and chicken bones was 12.4, 11.3 and 5.9 % w/w, calculated on dried basis, respectively. Total calcium determined by complexometric titration was 31.8, 28.1 and 32.2% w/w in hoki, giant seaperch and chicken bones, respectively. All samples contained carbonate and phosphate anion residues but gluconate, acetate and citrate were absent. The presence of calcium carbonate was confirmed by thermogravimetry. Results from all bones showed that limit tests for heavy metals, arsenic and iron complied with the USP standard,
whereas barium, chloride and sulfate conformed to the BP standard. The magnesium and alkali metals in giant seaperch bone were within the BP limit (1.5%), but those of hoki and chicken bone exceeded the limit