Somchai Chuan-udom, Samatchar Naksombut, and Jaruwan Boonsiri

pp. 569 - 578

Abstract

Various explant types-petiole, leaf lamina, spathe and spadix-of three varieties of anthurium were
cultured on modified Murashige and Skoog (MS) supplemented with 0.5 mg/l benzyladenine (BA) and
0.5 mg/l thidiazuron (TDZ). The results revealed that Tropicana variety gave the best average callus
formation from all explant types at 82%, followed by Champaign and Duang-sa-morn variety, respectively.
Among the explants, petiole resulted in the best callus formation at 82%, followed by leaf lamina (57%),
spadix (55%) and spathe (18%), respectively. Callus from leaf lamina gave the best shoot bud development in the presence of 0.25-0.5 mg/l BA. Petiole-derived callus gave response to BA at concentration of 0.5-
0.75 mg/l whereas spathe- and spadix-derived callus could not develop into shoot. Mass propagation of
cultivated variety of anthurium was routinely carried out as follows: leaf-derived callus was transferred
to shoot induction medium; promotion of healthy and elongation of the shoots was carried out in basal
MS-free medium; the shoots were then rooted in ½ MS hormone-free as root induction medium; acclimatization of plantlets was performed in coconut fiber as supporting planting material placed in 25-36-
hole plastic trays before transferring to growing pots.