Nunthiya Suwanpanya, Winyou Wongpratoom, Matha Wanapat, Suneerat Aiumlamai, Sunthon Wittayakun, and Chalong Wachirapakorn

pp. 697 - 706

Abstract

An experiment was performed to determine the influence of bovine nutrophils on in vitro phagocytosis and killing of Staphylococcus aureus after selenium (Se, selenium yeast) and vitamin E (vit E) was supplemented in heifers. Twelve healthycrossbred 75% Holstein-Friesian x Sahiwal heifers were divided into 4 groups in a 2x2 factorial arrangement in CRD. Heifers were supplemented organic selenium (selenium yeast) and vitamin E powder. The treatments were as follows; treatment 1) 3 mg Se 2,000 IU vit E /hd/d (3Se2E), treatment 2) 3 mg Se 4,000 IUvit E/hd/d (3Se 4E), treatment 3) 6 mg Se 2,000 IU vit E/hd/d (6Se2E), and treatment 4) 6 mg Se 4,000 IU vit E/hd/d (6Se 4E). The experiment comprised 3 periods: presupplementation (8 days), supplementation (8 days) and post-supplementation (8 days) periods. All heifers were offered concentrate (15% CP) at 4 kg/hd/d and rice straw hay ad libitum. Blood neutrophils were isolated from each heifer. Phagocytosis was determined by direct ingested count and killing of S. aureus by NBT reducting test. Phagocytosis and killing of S. aureus had a greater non-specific immune response during
the supplementation period than in the pre-supplementation period in all treatments (P<0.05). Supplementation of 3Se4E resulted in a greater number of white blood cells (54,900 cells/cu.mm) and neutrophils
(11,398 cells/cu.mm.) and improved phagocytosis (85%) and killing of S. aureus (47%) as compared to the pre-supplementation period.