Nuchjaree Watcharawongpaiboon and Julapark Chunwongse

pp. 1217 - 1223

Abstract

Development of microsatellite markers in Pumpkin (Cucurbita moschata L.) was performed using the biotin-streptavidin enrichment procedure. One hundred and thirty three clones were randomly selected. After sequence analysis of 31 randomly picked positive colonies, 100% of the colonies were found to contain microsatellite sequences, and 9 primer pairs were designed. Five of the primers tested could amplify pumpkins DNA and can be used for genetic purity testing of the commercial hybrids. This paper reports the first isolation and utilization of microsatellite markers in pumpkin.