Original Article |
2008, Vol.30, No.3, pp. 227-280
Nuclear DNA content of the pigeon orchid (Dendrobium crumenatum Sw.) with the analysis of flow cytometry
Upatham Meesawat, Theera Srisawat, Ladda Eksomtramage, and Kamnoon Kanchanapoom
pp. 227 - 280
Abstract
Nuclear DNA content for the adult plants grown in a greenhouse and in vitro young plantlets of the pigeon orchid (Dendrobium crumenatum Sw.) was analyzed using flow cytometry. The resulting 2C DNA values ranged from 2.30±0.14 pg to 2.43±0.06 pg. However, nuclear DNA ploidy levels of long-term in vitro plantlets were found to be triploid and tetraploid. These ploidy levels were confirmed by chromosome counting. Tetraploid individuals (2n = 4x = 76) had approximately two times DNA content than diploid (2n = 2x = 38) individuals. This variation may be due to prolonged cultivation and the presence of exogenous plant growth regulators.