Kunlaya Suwannarat and Charassri Nualsri

pp. 433 - 440

Abstract

A study was undertaken by RAPD to investigate genetic relationships among the following 4 Parkia species, Sator (Parkia speciosa Hassk.), Riang (P. timoriana Merr.), Khonkhong (P. leiophylla Kurz) and Lukding (P. sumatrana Miq.) and two plants of Parkia spp., namely Tong and Tien were also included. DNA from the leaf samples was isolated using CTAB. Of total 180 primers first screened, 8 primers were chosen to analyse genetic variation of 103 individual plants. A total of 125 amplified fragments was obtained from 8 primers with an average of 15.63 fragments per primer, of which 101
fragments (80.80%) were polymorphic. Some species-specific fragments were also obtained. With P. speciosa Hassk., 77 polymorphic fragments (68.75%) were obtained with an average of 14 fragments per primer, but no specific fragment could differentiate Sator khao from Sator dan. Results from the dendrogram revealed 5 groups of Parkia with similarity coefficients
ranging from 0.437-1.000. Tien was found to be in the same group as Riang while Tong could be separated from other Parkia with a close relationship to Khonkhong at a similarity value 0.725.