Shoot multiplication and rooting of Philodendronxanadu cultured in vitro
Yaowapha Jirakiattikul and Panadda Limpradithtanont
pp. 79 - 86
Abstract
Effects of plant growth regulators (PGR) at different concentrations on shoot multiplication and rooting of Philodendron xanadu were investigated. The experiments were arranged in a completely randomized design (CRD). Single shoots were cultured in vitro on MS medium supplemented with 0, 2 and 4 mg/l BA or in combination with 0.2 mg/l IAA for multiplication. The results showed that shoots cultured on the media supplemented with BA alone or with IAA proliferated more profusely than those cultured on medium without PGR. Explants produced a maximum number of 5.8 shoots on the medium supplemented with 4 mg/l BA but shoot length was significantly shorter. Rooting was induced on half-strength MS medium ( ½ MS), MS medium supplemented with 0, 0.5 and 1.0 mg/l IAA, NAA or IBA. Shoots rooted on all media but 100% rooting occurred on ½ MS and MS medium supplemented with 0.5 and 1.0 mg/l NAA or IBA. Survival was 100% after transplantation.
